to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.
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The database must undergo the upgrade process before the data can be accessed. The Browser also allows you to manage and process recorded data in the context of a single tube or panel as well as an entire experiment. After you draw a quadrant gate, the population hierarchy lists each quadrant as a subset of the T cells.
The printout includes the user name, instrument type, configuration name and details, and modification date. JoeSmith will be available as a login name the next time you log into the software, as you will see in the next section.
If automatic cleaning is disabled, run the fluidics startup procedure. Enable plate controls in the Plate window. Autointerval Gate button—draws an Interval gate automatically around a range of events on a dot or contour plot or histogram. Select a category from the menu; then enter specific information in the text field next to the menu. Enable the option to automatically delete manuzl after export.
Parallels and Parallels More information. Equal spacing tends to put most of the contour lines on the higher peaks representing larger numbers of events and might not show lower features.
Getting Started with BD FACSDiva Software
Messages are listed next to the time the event occurred. Define selections by clicking Add Value, selecting the value in the Value field, and entering the required text.
Figure Viewing instrument settings Instrument window: If more than one cell is selected, the change is made in all selected cells at the same time.
Workspace Toolbar The Workspace toolbar mznual near the top of the application window. To activate acquisition controls, click the icon next to the tube you want to acquire. Click in the same header again to reverse the sort order.
Duplicating Plots Duplicate a plot in one of the following ways: All parameters listed in the Parameters tab are available for ratio calculations. Select the generic name in the Name field and enter a new name.
All configuration management facssdiva such as set current, create, edit, and delete are done through the new Cytometer Setup and Tracking feature. We recommend providing this information so it can be used in keywords and in the User Tracking Log file.
See Using the Current Tube Pointer on page Use the search field above the Browser to find experiments or show fewer experiments in the Browser. See Changing Plot Parameters on page Browser toolbar Search field User icon Column divider Column header Open experiment Current tube pointer Closed experiment Create and access database elements in the Browser.
BD FACSDiva Files
Once a user has experiments, you can either disable the user name or delete their experiments and softwar delete the user name. BD expressly disclaims all implied warranties, including without limitation the implied warranties of merchantability, fitness for particular purpose, title and noninfringement. Drag and drop gates in the population hierarchy.
Don’t have an account? Data is listed by login name in a hierarchical view. Instrument settings can be applied at the rwference, specimen, or tube level. Click OK to dismiss the message, and either delete the duplicate user names or choose a different file to import.
Getting Started with BD FACSDiva Software – PDF
All selected fields are labeled with CD3 at once. See Adding Experiments on page For example, if you were performing an immunophenotyping experiment and wanted to collect data only for the lymphocytes, you could direct the software to collect 10, T-cell events for the stopping gate and record only events in the Lymphocyte storage gate.
This percentage is used as the maximum value for the y-axis, and changes automatically as the data displayed in the plot changes.
The software described in this guide is furnished under a software.